C2BBe1
C2BBe1(亦稱ATCC CRL-2102、Caco-2BBe)是對表皮生長因子呈陽性反應的人類結腸腺癌細胞系 ,最初是在1988年通過有限稀釋法,從一名72歲白人男性結腸腺癌患者的Caco-2細胞克隆出來而建系[1]。
特徴
C2BBe1細胞會形成極性且形態與人類結腸形態相似的單層細胞,故而早已應用於研究細菌的黏附和侵襲[2][3]。分離的頂端刷狀緣其實包含著微絨毛蛋白、絲束蛋白、蔗糖酶-異麥芽糖酶、 刷狀緣肌凝蛋白-1、胞襯蛋白及肌凝蛋白-2等物質[1]。C2BBe1細胞表達的刷狀緣肌凝蛋白-I的水平與人類腸上皮細胞相似,並且比親本Caco-2細胞株更加均勻[1],但是C2BBe1細胞在微絨毛的長度及部分刷狀緣蛋白表達水平方面仍然是不相同的。C2BBe1細胞目前可與小鼠派亞氏淋巴叢細胞共同培養,從而建立微皺褶細胞模型[4]。
參考資料
- ^ 1.0 1.1 1.2 Peterson, MD; Mooseker, MS. Characterization of the enterocyte-like brush border cytoskeleton of the C2BBe clones of the human intestinal cell line, Caco-2.. Journal of cell science. 1992-07,. 102 ( Pt 3): 581–600 [2019-12-11]. PMID 1506435.
- ^ Huang, Y; Adams, MC. An in vitro model for investigating intestinal adhesion of potential dairy propionibacteria probiotic strains using cell line C2BBe1.. Letters in applied microbiology. 2003, 36 (4): 213–6 [2019-12-11]. PMID 12641713. doi:10.1046/j.1472-765x.2003.01303.x.
- ^ Kops, SK; Lowe, DK; Bement, WM; West, AB. Migration of Salmonella typhi through intestinal epithelial monolayers: an in vitro study.. Microbiology and immunology. 1996, 40 (11): 799–811 [2019-12-11]. PMID 8985935. doi:10.1111/j.1348-0421.1996.tb01145.x.
- ^ Corr, S; Hill, C; Gahan, CG. An in vitro cell-culture model demonstrates internalin- and hemolysin-independent translocation of Listeria monocytogenes across M cells.. Microbial pathogenesis. 2006-12, 41 (6): 241–50 [2019-12-11]. PMID 17049432. doi:10.1016/j.micpath.2006.08.003.