硫代硫酸盐-柠檬酸盐-胆盐-蔗糖洋菜培养基

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硫代硫酸盐-柠檬酸盐-胆盐-蔗糖洋菜培养基(Thiosulfate-citrate-bile salts-sucrose agar,简称TCBS培养基),也称为弧菌选择培养基,是一种用来检验弧菌种类的培养基[1]无法发酵蔗糖的细菌,例如肠炎弧菌,在此培养基上的菌落颜色会呈现绿色;而能够发酵蔗糖的细菌菌落则呈现黄色。TCBS培养基的主要成分是硫代硫酸钠胆酸钠柠檬酸钠蔗糖。培养基还包含百里酚蓝溴百里酚蓝作为pH指示剂以显示培养细菌时的酸碱值变化。[2]

参考文献

  1. ^ Pfeffer, C.; Oliver, J.D. A comparison of thiosulphate-citrate-bile salts-sucrose (TCBS) agar and thiosulphate-chloride-iodide (TCI) agar for the isolation of Vibrio species from estuarine environments. Letters in Applied Microbiology. 2003, 36 (3): 150–151. PMID 12581373. doi:10.1046/j.1472-765X.2003.01280.x. 
  2. ^ Application TCBS Agar (Vibrio Selective Agar). website of the Merck KGaA. [2013-03-18].