MRC-5细胞
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MRC-5(全称英国医学研究委员会细胞株5、Medical Research Council cell strain-5)是由成纤维细胞组成的二倍体人类细胞系,该成纤维细胞来自于一个14周龄流产的白人男性胎儿的肺部组织,胞体呈单一梭形。1966年9月,研究人员从原始菌株的第7代增殖分裂中,分离出了该细胞系,并且已知MRC-5细胞在约42至46代的增殖分裂中,就会达到衰老[1][2]。在过氧化氢的体外诱导下,MRC-5细胞会出现早熟性衰老。MRC-5细胞可用于生产几种疫苗,包括麻疹腮腺炎德国麻疹混合疫苗、水痘疫苗和脊髓灰质炎疫苗等。
MRC-5细胞被很多科研单位和细胞培养工作者使用[3],因为其生产出的生物制品无外源因子、无外源性细胞残余去氧核糖核酸和无致瘤性。而且因具有广泛的病毒敏感谱而成为制备疫苗适宜的培养基质,然而因具有对培养基和牛血清等营养成分要求高,并且对剪应力敏感等的特点而具有培养难度大的缺点。有研究指出Cytodex 1[4]、Cytodex 2及Cytodex 3等均可应用于MRC-5细胞培养,其中Cytopore 2在细胞培养密度、代谢和形态等方面均比Cytodex 3更适合于MRC-5细胞的培养,使用其能进行高密度及大规模的培养[5][6][7]。
参考资料
- ^ Jacobs, JP; Jones, CM; Baille, JP. Characteristics of a human diploid cell designated MRC-5.. Nature. 1970-07-11, 227 (5254): 168–70 [2019-11-29]. PMID 4316953. doi:10.1038/227168a0.
- ^ Jacobs, JP. The status of human diploid cell strain MRC-5 as an approved substrate for the production of viral vaccines.. Journal of biological standardization. 1976-04, 4 (2): 97–9 [2019-11-29]. PMID 932048. doi:10.1016/0092-1157(76)90018-4.
- ^ Saeed, K; Pelosi, E. Comparison between turnaround time and cost of herpes simplex virus testing by cell culture and polymerase chain reaction from genital swabs.. International journal of STD & AIDS. 2010-04, 21 (4): 298–9 [2019-11-29]. PMID 20378906. doi:10.1258/ijsa.2009.009006.
- ^ Trabelsi, K; Majoul, S; Rourou, S; Kallel, H. Development of a measles vaccine production process in MRC-5 cells grown on Cytodex1 microcarriers and in a stirred bioreactor.. Applied microbiology and biotechnology. 2012-02, 93 (3): 1031–40 [2019-11-29]. PMID 21935589. doi:10.1007/s00253-011-3574-y.
- ^ Morandi, M; Bandinelli, L; Valeri, A. Growth of MRC-5 diploid cells on three types of microcarriers.. Experientia. 1982-06-15, 38 (6): 668–70 [2019-11-29]. PMID 7049716. doi:10.1007/bf01964083.
- ^ Varani, J; Bendelow, MJ; Chun, JH; Hillegas, WA. Cell growth on microcarriers: comparison of proliferation on and recovery from various substrates.. Journal of biological standardization. 1986-10, 14 (4): 331–6 [2019-11-29]. PMID 3558416. doi:10.1016/0092-1157(86)90020-x.
- ^ Forestell, SP; Kalogerakis, N; Behie, LA. The extended serial subculture of human diploid fibroblasts on microcarriers using a new medium supplement formulation.. Biotechnology and bioengineering. 1992-11, 40 (9): 1039–44 [2019-11-29]. PMID 18601212. doi:10.1002/bit.260400907.